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How To Use The Vimentin Antibody For Research

Vimentin is one of the main intermediate filament protein included in mesenchymal cells. Many believe that it is involved with intracellular transportation of proteins between the plasma membrane and nucleus. It can be used to stain the sarcoma of neural, fibroblast, and muscle origin, but usually works best in carcinomas that are negative. Lymphomas, melanomas, and vascular tumors can all be stained using this antibody.

More Information

The Vimentin antibody has a cloned called SP20, and the immunogen is the recombinant human protein of the same name. The isotype is the Rabbit IgG, and it has an undetermined epitope with a molecular weight of 53kDa.

Applications

This antibody can be used in Western Blotting, IHC, and Flow Cytometry applications.

The Western Blotting protocol begins by diluting the antibody using a ratio of 1:100 and incubating for one hour while at room temperature.

The Flow Cytometry protocol begins with diluting the antibody using a ratio of 1:100 and incubating for 30 minutes while at four degrees Celsius.

The IHC application requires you to use Formalin-fixed or paraffin-embedded tissues and deparaffinized slides.

You should dilute the antibody using a ratio of 1:200. There is no antigen retrieval method, though you should incubate for 30 minutes while at room temperature.

You will also find a pre-diluted formula that can be used for IHC applications only, and only when the protocols require a dilution rate of 1:200.

The positive control for IHC includes melanomas and sarcomas. The positive control for Western Blotting is the HeLa Cell Lysate, and Flow Cytometry controls include the HeLa Cell Line. Cellular localization will always occur in the cytoplasm.

The Vimentin antibody can be used to stain a variety of things and may transport proteins between the nucleus and membrane, as well. Visit Spring Bioscience at https://www.springbio.com today for more information.

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