Ki-67 is considered a nuclear protein and is expressed in proliferating cells. It is preferentially expressed during the late G1-, M-, S-, and G2-phases of the cell’s cycle, while cells in the quiescent phase are negative for the protein. The KI-67 antibody is designed to be used for research only and has no known clone. The immunogen is the synthetic peptide, which is derived from the C-terminus of the human Ki-67 protein. The isotype is the Rabbit IgG, and it has an undetermined epitope. The molecular weight is between 345 and 396 kDa.
The Ki-67 antibody works well with Immunohistochemistry applications. You should only use paraffin-embedded or Formalin-fixed tissues. Deparaffinized slides are also required and can be achieved with graded alcohols or xylene and its alternatives. When choosing the concentrated format, you should dilute the antibody using a ratio of one to 300. However, you can also find a pre-diluted version that is suitable.
To retrieve the antigen, you will need to boil the tissue section in a 10mM citrate buffer with a pH of 6.0 for at least ten minutes and allow it to cool to room temperature for at least 20 minutes. Incubation periods are 10 minutes at room temperature.
The positive control of the Ki-67 Antibody is the tonsil, breast carcinoma, and lymph node. Cellular localization occurs in the nucleus.
You can find concentrated versions that require dilution in formats of 0.1, 1.0, and 0.5 ml. Each of these is purified and come in a PBS/1% BSA buffer with a pH of 7.6 and less than 0.1% of sodium azide. You can also find a 7.0 ml pre-diluted version.
The Ki-67 antibody is designed to help you locate problems within the tonsil, lymph node and more. Visit Spring Bioscience to learn more and purchase this product.